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anti socs 2 antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti socs 2 antibody
    Anti Socs 2 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+socs2/SOCS2+Antibody/pm41864365-90-12-16
    Average 93 stars, based on 99 article reviews
    anti socs 2 antibody - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: SOCS2 alleviates traumatic brain injury-induced mitochondrial damage and parthanatos in endothelial cells by inhibiting the JAK2/STAT3 signaling pathway
    Article Snippet: Coimmunoprecipitation was performed by using a Pierce Co-IP kit (88804; Thermo Fisher, USA). .. First, RBE4 cell lysate was incubated with anti-SOCS2 (1:100; Cat# 2779; RRID: AB_2193157; CST, USA) or IgG (1:100; Cat# ab172730; RRID: AB_2687931; Abcam, UK) at 4 °C overnight and then incubated with protein A/G magnetic beads. ..

    Magnetic Beads:

    Article Title: SOCS2 alleviates traumatic brain injury-induced mitochondrial damage and parthanatos in endothelial cells by inhibiting the JAK2/STAT3 signaling pathway
    Article Snippet: Coimmunoprecipitation was performed by using a Pierce Co-IP kit (88804; Thermo Fisher, USA). .. First, RBE4 cell lysate was incubated with anti-SOCS2 (1:100; Cat# 2779; RRID: AB_2193157; CST, USA) or IgG (1:100; Cat# ab172730; RRID: AB_2687931; Abcam, UK) at 4 °C overnight and then incubated with protein A/G magnetic beads. ..

    Western Blot:

    Article Title: GM-CSF-miRNA-Jak2/Stat3 Signaling Mediates Chemotherapy-Induced Cancer Cell Stemness in Gastric Cancer
    Article Snippet: Protein bands were visualized using the Minichemi chemiluminescence Imaging System (Beijing Sage Creation Science Co., Ltd., China). .. The following antibodies were used for Western blot: anti- SOCS2 (2779T, Cell Signaling Technology), anti- JAK2 (3230T, Cell Signaling Technology), anti- p-JAK2 (4406T, Cell Signaling Technology), anti-STAT3 (9139T, Cell Signaling Technology), anti- p-STAT3 (9145T, Cell Signaling Technology), anti- OCT4 (2750S, Cell Signaling Technology), anti- NANOG (4903S, Cell Signaling Technology), anti- GAPDH (sc-47724, Santa Cruz), anti- KLF4 (sc-393462, Santa Cruz), anti- h-TERT (sc-377511, Santa Cruz), anti- GM-CSF (sc-32753, Santa Cruz) and anti- β-tubulin (ab18207, Abcam). .. Secondary antibodies (1:10,000) were HRP-linked anti-rabbit IgG (7074S, Cell Signaling Technology) and HRP-linked anti-mouse IgG (7076S, Cell Signaling Technology).

    Article Title: GM-CSF-miRNA-Jak2/Stat3 Signaling Mediates Chemotherapy-Induced Cancer Cell Stemness in Gastric Cancer.
    Article Snippet: Protein bands were visualized using the Minichemi chemiluminescence Imaging System (Beijing Sage Creation Science Co., Ltd., China). .. The following antibodies were used for Western blot: anti-SOCS2 (2779T, Cell Signaling Technology), anti-JAK2 (3230T, Cell Signaling Technology), anti-p-JAK2 (4406T, Cell Signaling Technology), anti-STAT3 (9139T, Cell Signaling Technology), anti-p-STAT3 (9145T, Cell Signaling Technology), anti-OCT4 (2750S, Cell Signaling Technology), anti-NANOG (4903S, Cell Signaling Technology), anti-GAPDH (sc-47724, Santa Cruz), anti-KLF4 (sc-393462, Santa Cruz), anti-h-TERT (sc-377511, Santa Cruz), antiGM-CSF (sc-32753, Santa Cruz) and anti-β-tubulin (ab18207, Abcam). .. Secondary antibodies (1:10,000) were HRP-linked anti-rabbit IgG (7074S, Cell Signaling Technology) and HRP-linked anti-mouse IgG (7076S, Cell Signaling Technology).

    Article Title: Epigenetic downregulation of Socs2 contributes to mutant N-Ras-mediated hematopoietic dysregulation
    Article Snippet: Anti-APC conjugated to paramagnetic microbeads were from Miltenyi Biotec. .. Antibodies for western blotting were anti-pStat5 (Y694), anti-Stat5 (4H1), anti-β-actin (8H10D10), anti-pJak2 (Tyr221), anti-Jak2, anti-pJAK1 (Tyr1034/1035) and anti-SOCS2 ( Table S3 ), all purchased from Cell Signaling Technology. .. Anti-GAPDH was from Santa Cruz.

    other:

    Article Title: Bone marrow mesenchymal stem cells-derived exosomal microRNA-185 represses ventricular remolding of mice with myocardial infarction by inhibiting SOCS2.
    Article Snippet: Objective: Recently, the function of microRNAs (miRNAs) has been clarified in human diseases, we aimed to identify the role of miR-185 in myocardial infarction (MI).. Methods: Bone marrow mesenchymal stem cells (BMSCs) were cultured, from which the exosomes were extracted.. MI mice models were established by coronary artery ligation and injected with transfected BMSCs.

    Article Title: SOCS2 alleviates traumatic brain injury-induced mitochondrial damage and parthanatos in endothelial cells by inhibiting the JAK2/STAT3 signaling pathway.
    Article Snippet: Coimmunoprecipitation was performed by using a Pierce Co-IP kit (88804; Thermo Fisher, USA).



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    FIGURE 1 | Expression levels of SOCS family members in LIHC and correlation with different clinicopathological features. Expression levels of SOCS family members in LIHC were determined by UCSC Xena database: compared to normal liver tissues, mRNA expression levels of <t>SOCS2</t> (p = 1.2E-15), SOCS3 (p = 1.3E-09), SOCS6 (p = 0.00014), and CISH (p = 0.0011) decreased in HCC, while expression levels of SOCS4 (p = 1.1e-06), SOCS5 (p = 2.4E-12), and SOCS7 (p = 2.22E-16) significantly increased.
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    FIGURE 1 | Expression levels of SOCS family members in LIHC and correlation with different clinicopathological features. Expression levels of SOCS family members in LIHC were determined by UCSC Xena database: compared to normal liver tissues, mRNA expression levels of <t>SOCS2</t> (p = 1.2E-15), SOCS3 (p = 1.3E-09), SOCS6 (p = 0.00014), and CISH (p = 0.0011) decreased in HCC, while expression levels of SOCS4 (p = 1.1e-06), SOCS5 (p = 2.4E-12), and SOCS7 (p = 2.22E-16) significantly increased.
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    FIGURE 1 | Expression levels of SOCS family members in LIHC and correlation with different clinicopathological features. Expression levels of SOCS family members in LIHC were determined by UCSC Xena database: compared to normal liver tissues, mRNA expression levels of <t>SOCS2</t> (p = 1.2E-15), SOCS3 (p = 1.3E-09), SOCS6 (p = 0.00014), and CISH (p = 0.0011) decreased in HCC, while expression levels of SOCS4 (p = 1.1e-06), SOCS5 (p = 2.4E-12), and SOCS7 (p = 2.22E-16) significantly increased.
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    Image Search Results


    FIGURE 1 | Expression levels of SOCS family members in LIHC and correlation with different clinicopathological features. Expression levels of SOCS family members in LIHC were determined by UCSC Xena database: compared to normal liver tissues, mRNA expression levels of SOCS2 (p = 1.2E-15), SOCS3 (p = 1.3E-09), SOCS6 (p = 0.00014), and CISH (p = 0.0011) decreased in HCC, while expression levels of SOCS4 (p = 1.1e-06), SOCS5 (p = 2.4E-12), and SOCS7 (p = 2.22E-16) significantly increased.

    Journal: Cancer reports (Hoboken, N.J.)

    Article Title: Expression Characteristics, Immune Signature, and Prognostic Value of the SOCS Family Identified by Multiomics Integrative Analysis in Liver Cancer.

    doi: 10.1002/cnr2.2161

    Figure Lengend Snippet: FIGURE 1 | Expression levels of SOCS family members in LIHC and correlation with different clinicopathological features. Expression levels of SOCS family members in LIHC were determined by UCSC Xena database: compared to normal liver tissues, mRNA expression levels of SOCS2 (p = 1.2E-15), SOCS3 (p = 1.3E-09), SOCS6 (p = 0.00014), and CISH (p = 0.0011) decreased in HCC, while expression levels of SOCS4 (p = 1.1e-06), SOCS5 (p = 2.4E-12), and SOCS7 (p = 2.22E-16) significantly increased.

    Article Snippet: Antibodies used in this study included anti- SOCS2 (1:2000, Proteintech), beta- actin (1:2000, Proteintech), and horseradish peroxids- mediated secondary goat anti- mouse (1:5000, Biosharp).

    Techniques: Expressing

    FIGURE 4 | Survival analysis of SOCS family members in LIHC patients: prognostic impact on overall survival (OS). The expression levels of SOCS2 (p < 0.0001), SOCS4 (p = 0.0064), SOCS5 (p = 0.034), SOCS6 (p = 0.037), and CISH (p = 0.034) were found to be significantly associated with OS in HCC patients.

    Journal: Cancer reports (Hoboken, N.J.)

    Article Title: Expression Characteristics, Immune Signature, and Prognostic Value of the SOCS Family Identified by Multiomics Integrative Analysis in Liver Cancer.

    doi: 10.1002/cnr2.2161

    Figure Lengend Snippet: FIGURE 4 | Survival analysis of SOCS family members in LIHC patients: prognostic impact on overall survival (OS). The expression levels of SOCS2 (p < 0.0001), SOCS4 (p = 0.0064), SOCS5 (p = 0.034), SOCS6 (p = 0.037), and CISH (p = 0.034) were found to be significantly associated with OS in HCC patients.

    Article Snippet: Antibodies used in this study included anti- SOCS2 (1:2000, Proteintech), beta- actin (1:2000, Proteintech), and horseradish peroxids- mediated secondary goat anti- mouse (1:5000, Biosharp).

    Techniques: Expressing

    FIGURE 5 | (A) Cox regression analysis indicated: SOCS2 and SOCS4 were associated with the OS of patients with LIHC. (B) Through VENN analysis, 64 related genes were found related to ferroptosis. (C) The GSEA analysis showed that SOCS family members is closely related to ferroptosis genes. (D) Western blot revealed that SOCS2 overexpression plasmids lead to an upregulation of SOCS2. (E) Analysis of the PCR revealed that the expression of SOCS2 was significantly upregulated in the SOCS2 overexpression plasmids. (F) SOCS2 inhibited cell proliferation by CCK-8 assay. (G) Over- expression of SOCS2 inhibited migration and invasion in vitro.(H) The migratory cell per field in the SOCS2 overexpression group is less than that in the vector group (498 ± 21 vs. 1380 ± 65, p < 0.05). The invasive cell per field in the SOCS2 over-expression group is less than that in the vector group (98 ± 8 vs. 373 ± 41, p < 0.05). Data are presented as mean ± SEM (n = 4). **p < 0.01, ***p < 0.001. Scale bar, 400 um.

    Journal: Cancer reports (Hoboken, N.J.)

    Article Title: Expression Characteristics, Immune Signature, and Prognostic Value of the SOCS Family Identified by Multiomics Integrative Analysis in Liver Cancer.

    doi: 10.1002/cnr2.2161

    Figure Lengend Snippet: FIGURE 5 | (A) Cox regression analysis indicated: SOCS2 and SOCS4 were associated with the OS of patients with LIHC. (B) Through VENN analysis, 64 related genes were found related to ferroptosis. (C) The GSEA analysis showed that SOCS family members is closely related to ferroptosis genes. (D) Western blot revealed that SOCS2 overexpression plasmids lead to an upregulation of SOCS2. (E) Analysis of the PCR revealed that the expression of SOCS2 was significantly upregulated in the SOCS2 overexpression plasmids. (F) SOCS2 inhibited cell proliferation by CCK-8 assay. (G) Over- expression of SOCS2 inhibited migration and invasion in vitro.(H) The migratory cell per field in the SOCS2 overexpression group is less than that in the vector group (498 ± 21 vs. 1380 ± 65, p < 0.05). The invasive cell per field in the SOCS2 over-expression group is less than that in the vector group (98 ± 8 vs. 373 ± 41, p < 0.05). Data are presented as mean ± SEM (n = 4). **p < 0.01, ***p < 0.001. Scale bar, 400 um.

    Article Snippet: Antibodies used in this study included anti- SOCS2 (1:2000, Proteintech), beta- actin (1:2000, Proteintech), and horseradish peroxids- mediated secondary goat anti- mouse (1:5000, Biosharp).

    Techniques: Western Blot, Over Expression, Expressing, CCK-8 Assay, Migration, In Vitro, Plasmid Preparation